iix myosin heavy chain (Developmental Studies Hybridoma Bank)
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iix myosin heavy chain
Iix Myosin Heavy Chain, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 97/100, based on 673 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/type+iix/anti-Myosin+heavy+chain+Type+I/bio_rxiv__64898__2026__04__02__716074-237-10-21
Average 97 stars, based on 673 article reviews
Iix Myosin Heavy Chain, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 97/100, based on 673 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/type+iix/anti-Myosin+heavy+chain+Type+I/bio_rxiv__64898__2026__04__02__716074-237-10-21
Average 97 stars, based on 673 article reviews
iix myosin heavy chain - by Bioz Stars,
2026-10
97/100 stars
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Immunohistochemical staining:Article Title: The effects of environmental enrichment on voluntary physical activity and muscle mass gain in growing rats Article Snippet: .. For the immunohistochemical assessment of the muscle fiber-type distribution and fiber-type-specific CSA, slides were incubated for 1 h at room temperature in primary antibodies against myosin heavy chain type I (mouse IgG, 1:40, Developmental Studies Hybridoma Bank (DSHB), University of Iowa), type IIa (mouse IgG, 1:1000, DSHB, University of Iowa), type IIb (mouse IgG, 1:100, DSHB, University of Iowa), and Incubation:Article Title: The effects of environmental enrichment on voluntary physical activity and muscle mass gain in growing rats Article Snippet: .. For the immunohistochemical assessment of the muscle fiber-type distribution and fiber-type-specific CSA, slides were incubated for 1 h at room temperature in primary antibodies against myosin heavy chain type I (mouse IgG, 1:40, Developmental Studies Hybridoma Bank (DSHB), University of Iowa), type IIa (mouse IgG, 1:1000, DSHB, University of Iowa), type IIb (mouse IgG, 1:100, DSHB, University of Iowa), and Article Title: Voluntary wheel running improves molecular and functional deficits in a murine model of facioscapulohumeral muscular dystrophy Article Snippet: .. Briefly, slides were blocked in 1% bovine-serum albumin, 1% goat normal serum (Vector Laboratories S-1000-20, Newark, CA, USA), and 3.6% M.O.M blocking reagent (Vector Laboratories, MKB-2213-1) in PBS for 1 hour at room temperature, then incubated with primary antibodies against myosin heavy chain type 1 (BA-D5, 3.05 μg /ml), type IIa (SC-71, 0.85 μg /ml), Article Title: Activation of mitophagy and proteasomal degradation confers resistance to developmental defects in postnatal skeletal muscle Article Snippet: .. Slides were incubated at room temperature with antibodies against type IIA (SC-71, DSHB), Article Title: Exercise and ageing impact the kynurenine/tryptophan pathway and acylcarnitine metabolite pools in skeletal muscle of older adults. Article Snippet: Steady state oxygen flux was determined for each respiratory state using DatLab 5 (Oroboros Instruments) and normalized to fibre bundle wet weight. .. For muscle fibre type, sections were blocked in 10% goat serum and incubated overnight with primary antibodies directed toward type I [BA-F8 (IgG2b); Developmental Studies Hybridoma Bank (DSHB), Iowa City, IA, USA], type IIa [SC-71 (IgG1); DSHB] and Article Title: Mitophagy is required to protect against excessive skeletal muscle atrophy following hindlimb immobilization. Article Snippet: .. Slides were incubated at room temperature with antibodies against type IIA (SC-71, DSHB), Article Title: Mitophagy is required to protect against excessive skeletal muscle atrophy following hindlimb immobilization Article Snippet: .. Slides were incubated at room temperature with antibodies against type IIA (SC-71, DSHB), Article Title: Activation of mitophagy and proteasomal degradation confers resistance to developmental defects in postnatal skeletal muscle. Article Snippet: .. Slides were incubated at room temperature with antibodies against type IIA (SC-71, DSHB), Blocking Assay:Article Title: Voluntary wheel running improves molecular and functional deficits in a murine model of facioscapulohumeral muscular dystrophy Article Snippet: .. Briefly, slides were blocked in 1% bovine-serum albumin, 1% goat normal serum (Vector Laboratories S-1000-20, Newark, CA, USA), and 3.6% M.O.M blocking reagent (Vector Laboratories, MKB-2213-1) in PBS for 1 hour at room temperature, then incubated with primary antibodies against myosin heavy chain type 1 (BA-D5, 3.05 μg /ml), type IIa (SC-71, 0.85 μg /ml), Staining:Article Title: An alternative mechanism for skeletal muscle dysfunction in long-term post-viral lung disease. Article Snippet: Sections were washed and labeled with a combination of unconjugated rabbit anti-laminin antibody (Abcam Cat. No. ab11575, RRID:AB_298179) (1:400) that has been used previously for specific staining of basal lamina in skeletal muscle (35–41). .. In addition, these sections were stained with mouse antibody against a specific type of myosin heavy chain (MHC): Type I (DSHB Cat. No. BA-F8, RRID:AB_10572253), Type IIA (DSHB Cat. No.SC-71, RRID:AB_2147165), Immunopeptidomics:Article Title: An alternative mechanism for skeletal muscle dysfunction in long-term post-viral lung disease. Article Snippet: Sections were washed and labeled with a combination of unconjugated rabbit anti-laminin antibody (Abcam Cat. No. ab11575, RRID:AB_298179) (1:400) that has been used previously for specific staining of basal lamina in skeletal muscle (35–41). .. In addition, these sections were stained with mouse antibody against a specific type of myosin heavy chain (MHC): Type I (DSHB Cat. No. BA-F8, RRID:AB_10572253), Type IIA (DSHB Cat. No.SC-71, RRID:AB_2147165), |
![A: Cross-sections (10 µm) of bovine longissimus dorsi muscle biopsy samples were stained with a primary antibody against dystrophin [MANDYS1(3B7)s, MIgG2a, 1:10] to label the label the myofiber boundaries, together with a cocktail of mouse monoclonal primary antibodies against MyHC type I (BA-D5c, MIgG2b, 1:100) and MyHC type IIA (SC-71c, MIgG1, 1:100). Primary antibody staining was visualized using Alexa Fluor-conjugated secondary antibodies (1: 500) including Goat Anti-Mouse IgG2a Alexa Fluor 647 (to detect dystrophin), Goat Anti-Mouse IgG1 Alexa Fluor 488 (to detect SC-71), and Goat Anti-Mouse IgG2b Alexa Fluor 555 (to detect BA-D5). Dystrophin, BA-D5, and SC-71 staining was pseudo colored white, red, and green, respectively. B: Serial cross-sections from the same bovine longissimus dorsi muscle biopsy sample shown in panel A were stained with a primary antibody against dystrophin [MANDYS1(3B7)s, MIgG2a, 1:10] to label the label the myofiber boundaries in combination with a cocktail of primary antibodies against MyHC type IIX (6H1s, MIgM, 1:10) and all MyHC isoforms except for type IIX (BF-35c, MIgG1, 1:100). Primary antibody staining was visualized using Alexa Fluor-conjugated secondary antibodies (1:500) including Goat Anti-Mouse IgG2a Alexa Fluor 647 (to detect dystrophin), Goat Anti-Mouse IgG1 Alexa Fluor 488 (to detect BF-35), and Goat Anti-Mouse IgM Alexa Fluor 555 (to detect 6H1). Dystrophin, BF-35, and 6H1 staining were pseudo colored white, red, and green, respectively. Scale bars are 200 µm.](https://bio-rxiv-images-cdn.bioz.com/dois_ending_with_34/10__64898_slash_2025__12__18__695234/10__64898_slash_2025__12__18__695234___F2.large.jpg)
